Effective Light-Induced Hydroxylamine Reactions Occur with C13=C14 Nonisomerizable Bacteriorhodopsin Pigments
نویسندگان
چکیده
منابع مشابه
Photoreduction of bacteriorhodopsin Schiff base at low humidity. A study with C13=C14 nonisomerizable artificial pigments.
The retinal protonated Schiff base of bacteriorhodopsin is photoreactive to reducing agents such as NaBH4. In the present work we have studied the effect of different protein hydration levels on the photoreductive reaction, as well as the consequences of preventing isomerization around the critical C13=C14 retinal double bond. It was revealed that the rate of light-induced NaBH4 reaction can be...
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The structure of bacteriorhodopsin based on electron microscopy (EM) studies, as provided in Henderson et al. (1990), is refined using molecular dynamics simulations. The work is based on a previously refined and simulated structure which had added the interhelical loops to the EM model of bR. The present study applies an all-atom description to this structure and constraints to the original He...
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متن کاملPhotoisomerization, energy storage, and charge separation: a model for light energy transduction in visual pigments and bacteriorhodopsin.
A simple model for the early events in visual pigments and bacteriorhodopsin is proposed. The model makes use of the likelihood that a negatively charged amino acid forms a salt bridge with the positively charged nitrogen of the retinylic chromophore. The photochemical event is a cis-trans isomerization in visual pigments and a trans-cis isomerization in bacteriorhodopsin, which in each case cl...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1998
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(98)77526-5